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rabbit anti-limp2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rabbit anti-limp2
    Histochemical and immunohistochemical staining of cornea and retina in unaffected and MPS I affected dogs. Alcian blue (A–B), luxol fast blue (C–D), and lysosomal integral membrane protein 2 <t>(LIMP2)</t> immunohistochemistry (E–F), 400x. (A) Alcian blue (AB) stained cornea from a 1-year-old unaffected control dog. B) AB stained cornea from a 1-year-old MPS I affected dog. Markedly vacuolated stromal cells contain AB positive storage material (arrow). (C) Luxol fast blue (LFB) stained retina from a 2.1-year-old unaffected control dog. (D) LFB stained retina from a 1-year-old MPS I affected dog. Rare retinal ganglion cells contain LFB positive storage material (arrow). (E) LIMP2 immunoreactivity in the retina of a 2.1-year-old unaffected control. (F) Retinal LIMP2 immunoreactivity in a 2.1-year-old MPS I affected dog. There is increased immunoreactivity in MPS I affected retinas, predominantly in the retinal ganglion cell layer (arrow)
    Rabbit Anti Limp2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-limp2/pmc11372224-60-8-11
    Average 90 stars, based on 1 article reviews
    rabbit anti-limp2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I"

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I

    Journal: Journal of inherited metabolic disease

    doi: 10.1002/jimd.12587

    Histochemical and immunohistochemical staining of cornea and retina in unaffected and MPS I affected dogs. Alcian blue (A–B), luxol fast blue (C–D), and lysosomal integral membrane protein 2 (LIMP2) immunohistochemistry (E–F), 400x. (A) Alcian blue (AB) stained cornea from a 1-year-old unaffected control dog. B) AB stained cornea from a 1-year-old MPS I affected dog. Markedly vacuolated stromal cells contain AB positive storage material (arrow). (C) Luxol fast blue (LFB) stained retina from a 2.1-year-old unaffected control dog. (D) LFB stained retina from a 1-year-old MPS I affected dog. Rare retinal ganglion cells contain LFB positive storage material (arrow). (E) LIMP2 immunoreactivity in the retina of a 2.1-year-old unaffected control. (F) Retinal LIMP2 immunoreactivity in a 2.1-year-old MPS I affected dog. There is increased immunoreactivity in MPS I affected retinas, predominantly in the retinal ganglion cell layer (arrow)
    Figure Legend Snippet: Histochemical and immunohistochemical staining of cornea and retina in unaffected and MPS I affected dogs. Alcian blue (A–B), luxol fast blue (C–D), and lysosomal integral membrane protein 2 (LIMP2) immunohistochemistry (E–F), 400x. (A) Alcian blue (AB) stained cornea from a 1-year-old unaffected control dog. B) AB stained cornea from a 1-year-old MPS I affected dog. Markedly vacuolated stromal cells contain AB positive storage material (arrow). (C) Luxol fast blue (LFB) stained retina from a 2.1-year-old unaffected control dog. (D) LFB stained retina from a 1-year-old MPS I affected dog. Rare retinal ganglion cells contain LFB positive storage material (arrow). (E) LIMP2 immunoreactivity in the retina of a 2.1-year-old unaffected control. (F) Retinal LIMP2 immunoreactivity in a 2.1-year-old MPS I affected dog. There is increased immunoreactivity in MPS I affected retinas, predominantly in the retinal ganglion cell layer (arrow)

    Techniques Used: Immunohistochemical staining, Staining, Membrane, Immunohistochemistry, Control

    Mean retinal LIMP2 immunoreactivity in 6 unaffected and 6 MPS I affected dogs of clinical age. (A) Mean LIMP2 immunolabeling is significantly increased ( p -value 0.0094) in MPS I dogs compared to unaffected controls. Error bars represent standard error of the mean. (B) Individual animal data. LIMP2 measurements were taken from four regions of the retina. Each bar represents the mean and standard deviation. At all timepoints, LIMP2 immunoreactivity is increased compared to similarly aged controls but does not appear to increase with age. X-axis is age in years
    Figure Legend Snippet: Mean retinal LIMP2 immunoreactivity in 6 unaffected and 6 MPS I affected dogs of clinical age. (A) Mean LIMP2 immunolabeling is significantly increased ( p -value 0.0094) in MPS I dogs compared to unaffected controls. Error bars represent standard error of the mean. (B) Individual animal data. LIMP2 measurements were taken from four regions of the retina. Each bar represents the mean and standard deviation. At all timepoints, LIMP2 immunoreactivity is increased compared to similarly aged controls but does not appear to increase with age. X-axis is age in years

    Techniques Used: Immunolabeling, Standard Deviation

    Related Articles

    Incubation:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).

    Immunohistochemical staining:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).

    Staining:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).

    Membrane:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).

    Immunohistochemistry:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).

    Control:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).

    Immunolabeling:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).

    Standard Deviation:

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I
    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Article Title: The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease
    Article Snippet: The following primary antibodies were used: rabbit anti-LRRK2 (abcam, UDD3 ab170993, 1:2000), rabbit anti-pS1292-LRRK2 (abcam, ab206035, 1:2000), rabbit anti-pS935-LRRK2 [UDD2] (abcam, ab172382, 1:1000), rabbit anti-LRRK2 (abcam, c41-2 ab133474, 1:1000), rabbit anti-Glucocerebrosidase (Sigma, G4171, 1:1000), rabbit anti-Rab29 (abcam, ab199644, 1:1000), rabbit anti-pT71-Rab29 (abcam, ab241062), mouse anti-γ tubulin (Sigma, T5326-25UL), mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific, AB_2182836, 1:1000), rabbit anti-SOD1 (Santa Cruz, sc11407, 1:1000), rabbit anti-human LAMP2A (abcam, ab23322, 1:1000), mouse anti-LAMP1 (sc-20011).



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    Histochemical and immunohistochemical staining of cornea and retina in unaffected and MPS I affected dogs. Alcian blue (A–B), luxol fast blue (C–D), and lysosomal integral membrane protein 2 (LIMP2) immunohistochemistry (E–F), 400x. (A) Alcian blue (AB) stained cornea from a 1-year-old unaffected control dog. B) AB stained cornea from a 1-year-old MPS I affected dog. Markedly vacuolated stromal cells contain AB positive storage material (arrow). (C) Luxol fast blue (LFB) stained retina from a 2.1-year-old unaffected control dog. (D) LFB stained retina from a 1-year-old MPS I affected dog. Rare retinal ganglion cells contain LFB positive storage material (arrow). (E) LIMP2 immunoreactivity in the retina of a 2.1-year-old unaffected control. (F) Retinal LIMP2 immunoreactivity in a 2.1-year-old MPS I affected dog. There is increased immunoreactivity in MPS I affected retinas, predominantly in the retinal ganglion cell layer (arrow)

    Journal: Journal of inherited metabolic disease

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I

    doi: 10.1002/jimd.12587

    Figure Lengend Snippet: Histochemical and immunohistochemical staining of cornea and retina in unaffected and MPS I affected dogs. Alcian blue (A–B), luxol fast blue (C–D), and lysosomal integral membrane protein 2 (LIMP2) immunohistochemistry (E–F), 400x. (A) Alcian blue (AB) stained cornea from a 1-year-old unaffected control dog. B) AB stained cornea from a 1-year-old MPS I affected dog. Markedly vacuolated stromal cells contain AB positive storage material (arrow). (C) Luxol fast blue (LFB) stained retina from a 2.1-year-old unaffected control dog. (D) LFB stained retina from a 1-year-old MPS I affected dog. Rare retinal ganglion cells contain LFB positive storage material (arrow). (E) LIMP2 immunoreactivity in the retina of a 2.1-year-old unaffected control. (F) Retinal LIMP2 immunoreactivity in a 2.1-year-old MPS I affected dog. There is increased immunoreactivity in MPS I affected retinas, predominantly in the retinal ganglion cell layer (arrow)

    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Techniques: Immunohistochemical staining, Staining, Membrane, Immunohistochemistry, Control

    Mean retinal LIMP2 immunoreactivity in 6 unaffected and 6 MPS I affected dogs of clinical age. (A) Mean LIMP2 immunolabeling is significantly increased ( p -value 0.0094) in MPS I dogs compared to unaffected controls. Error bars represent standard error of the mean. (B) Individual animal data. LIMP2 measurements were taken from four regions of the retina. Each bar represents the mean and standard deviation. At all timepoints, LIMP2 immunoreactivity is increased compared to similarly aged controls but does not appear to increase with age. X-axis is age in years

    Journal: Journal of inherited metabolic disease

    Article Title: Clinical and pathological characterization of ophthalmic disease in a canine model of mucopolysaccharidosis type I

    doi: 10.1002/jimd.12587

    Figure Lengend Snippet: Mean retinal LIMP2 immunoreactivity in 6 unaffected and 6 MPS I affected dogs of clinical age. (A) Mean LIMP2 immunolabeling is significantly increased ( p -value 0.0094) in MPS I dogs compared to unaffected controls. Error bars represent standard error of the mean. (B) Individual animal data. LIMP2 measurements were taken from four regions of the retina. Each bar represents the mean and standard deviation. At all timepoints, LIMP2 immunoreactivity is increased compared to similarly aged controls but does not appear to increase with age. X-axis is age in years

    Article Snippet: Sections were then incubated overnight at 4°C with rabbit anti-LIMP2 (PA3–16802, Invitrogen; 1:500).

    Techniques: Immunolabeling, Standard Deviation